Peculiarities of IBD Clinical Symptoms Manifestation in Muc2- Mice During Siblings Cohousing

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Abstract

Clinical symptoms of inflammatory bowel diseases (IBD) are one of the indicators of the severity of the disease. A clear understanding of the variability in the manifestation of clinical symptoms and the time of their manifestation is extremely important to monitor the effect of treatment methods on the course of inflammatory bowel diseases. One of the effective models of inflammatory bowel diseases is the Muc2- mouse line, which is characterized by the manifestation of similar clinical symptoms of IBD as in humans. In this regard, we decided to study the features of IBD symptoms in male and female Muc2- line mice, so that in the future we could create a protocol for assessing the effect of drugs on the development and course of the disease. In our work, we observed that clinical symptoms tend to manifest themselves differently depending on the sex of the animal. Thus, rectal prolapse with bleeding was manifested mainly in males of the Muc2- lineage, while signs of exhaustion were characteristic mainly of females.

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About the authors

K. S. Milutinovich

Lomonosov Moscow State University

Author for correspondence.
Email: Milutinovic.Ksenija@yandex.ru
Russian Federation, Moscow

V. S. Popov

Lomonosov Moscow State University

Email: Milutinovic.Ksenija@yandex.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Example of prolapse score in points. (a) - Normal; (b) - 1 point; (c), (d) and (e) - 2 points; (f) and (g) - 3 points; (h) and (i) - 5 points.

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3. Fig. 2. Degree of emaciation assessed in points. Measured between the 13th and 23rd week of animal life and presented as the mean depletion score of the animal during the measurement period. Mann-Whitney, * - p < 0.05; N1(f muc) = 3, N2(f wt) = 4, N3(f het) = 4, N4(m m muc) = 3, N5(m wt) = 4, N6(m het) = 6.

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4. Fig. 3. Dynamics of exhaustion in females (data are presented as median and interquartile range, on the axis of ordinates - exhaustion in points). N1(f muc) = 3, N2(f wt) = 4, N3(f het) = 4.

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5. Fig. 4. Extent of rectal prolapse and bleeding, assessed in scores between the 13th and 23rd week of animal life and presented as the mean rectal prolapse manifestation score with or without bleeding in the animal during the measurement period. Mann-Whitney, * - p < 0.05; ** - p < 0.01; N1(f muc) = 3, N2(f wt) = 4, N3(f het) = 4, N4(m muc) = 3, N5(m wt) = 4, N6(m het) = 6.

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6. Fig. 5. Dynamics of rectal prolapse and bleeding in males (median values) expressed as prolapse manifestation scores. N4(m muc) = 3, N5(m wt) = 4, N6(m het) = 6.

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7. Fig. 6. Dynamics of body weight gain from the 7th to the 25th week of life. Data are presented in the form of average body weight of all animals of a certain sex and genotype at the study point. N1(f muc) = 3, N2(f wt) = 4, N4(m muc) = 3, N5(m wt) = 4.

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